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KMID : 0368420090520060560
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Journal of Plant Biology
2009 Volume.52 No. 6 p.560 ~ p.560
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Combinatorial Interactions of Two cis-Acting Elements, AT-Rich Regions and HSEs, in the Expression of Tomato Lehsp23.8 upon Heat and Non-Heat Stresses
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Yi Shu-Ying
Liu Jian
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Abstract
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We previously cloned and analyzed the 1,893-bp promoter region (?1,915 to ?23) of the tomato (Lycopersicon esculentum) Lehsp23.8 gene, whose expression is induced by treatment with high or low temperatures, heavy metal, or abscisic acid (ABA). In our present work, we examined how this expression is regulated. A comprehensive quantitative promoter deletion and base-substitution analysis was conducted under various environmental conditions. The proximal region (?565 to ?23 bp) of the Lehsp23.8 promoter harbors cis-regulatory elements that conferred high levels of heat-induced expression in transgenic tobacco. Mutation of the five proximal HSEs (HSE1 to 5) of that promoter led to an absence of heat inducibility. The AT-rich regions between ?255 bp and ?565 bp (AT-rich1 to 4) in the promoter might serve as enhancers for such heat-induced expression. Deletion and HSE mutation analysis indicated that other cis-acting elements also function in response to low temperature, heavy metal, and ABA and that HSE1 to 5 act at least as cis-acting elements in multiple-stress responses of Lehsp23.8. These results reveal that those five proximal HSEs and AT-rich regions function interdependently in the expression of Lehsp23.8 in response to non-heat stresses. Furthermore, the putative elements CRT/DRE, AP-1, and ABRE in that promoter are not required for multiple-stress induction.
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KEYWORD
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Deletion analysis, GUS activity, Lehsp23.8, Promoter, Stress
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